Determination of Ferulic Acid and Gastrodin in Drug Delivery System of Dachuanxiong by HPLC/DAD
- VernacularTitle:HPLC-DAD法同时测定大川芎复方释药系统中阿魏酸与天麻素的含量
- Author:
Yuanfeng WEI
;
Ning ZHANG
;
Yi FENG
- Publication Type:Journal Article
- Keywords:
drug delivery system of Dachuanxiong;
gastrodin;
ferulic acid;
HPLC/DAD;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a RP-HPLC method for determining gastrodin and ferulic acid in drug delivery system of Dachuanxiong simultaneously. Methods ODS-2 Hypersil column was used with methanol-1% glacial acetic acid as solution gradient elution. The flow rate was 1 mL/min and column temperature was 25 ℃. The wavelength of detector of gastrodin was 270 nm, and ferulic acid was 322 nm. Results Gastrodin and ferulic acid can be separated well with other components within 40 minutes. The linear range of gastrodin was 0.092～1.840 ?g (r =1.000 0), and ferulic acid was 0.122～2.440 ?g (r =1.000 0). The average recovery rate of gastrodin was 99.50% with RSD=1.23% (n =5), and ferulic acid was 101.5% with RSD=1.52% (n=5). Conclusion The method is simple, rapid, accurate and reliable, and can be used for determining gastrodin and ferulic acid in drug delivery system of Dachuanxiong simultaneously.
