Determination of Hydroxysafflor Yellow A Content in Drug Pair of Flos Carthami and Semen Persicae in Different Ratios by HPLC
- VernacularTitle:高效液相色谱法测定桃仁红花配伍药对中羟基红花黄色素A含量的变化
- Author:
Rong ZHANG
;
Tao WANG
;
Qide LIU
;
Shaojie CHEN
- Publication Type:Journal Article
- Keywords:
SEMEN PERSICAE/ chemistry;
FLOS CARTHAMI/ chemistry;
HYDROXYSAFFLOR YELLOW A / analysis;
CHROMATOGRAPHY,HIGH PRESSURE LIQUID
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of compatibility of Flos Carthami and Semen Persicae in different ratios on the contents of hydroxysafflor yellow A(HSYA) in their water decoction.Methods Reverse-phase HPLC was performed on a Luna C18 column(4.6 mm?150 mm,5?m) at 30℃.The mobile phase consisted of methanol-0.12 mmol?L-1 phosphate solution(28∶72,V/V) at the flow rate of 1.0 mL? min-1.The detector was operated at wavelength of 403nm.Results A good linearity of HSYA was obtained in the range of 4.0～160.0?g?mL-1(Y=25 396X+40 247,r=0.999 7),the average recovery was 94.76% and the RSD(n=6) was 2.93%.The amount of HSYA increased with the ratio of Flos Carthami,but had no correlation with the ratio of Semen Persicae.Conclusion The HPLC method for determination of HSYA is simple,fast and accurate.It can be used for the quality control of Flos Carthami and its compound preparations.Semen Persicae has no effect on the extraction of HSYA.
