The influence of BMP_9 in myocardiocytes differentiation of stem cell in vitro

VernacularTitle:BMP_9对C_3H10T_(1/2)干细胞向心肌样细胞分化的影响
Author: Jintong TAN
Publication Type:Journal Article
Keywords: BMP9; C3H10T1/2stemcell; Differentiation; Cardiomyocytes
From: Journal of Chongqing Medical University 2003;0(06):-
CountryChina
Language:Chinese
Abstract: Objective:ToexploreinfluenceofBMP9inmyocardiocytesdifferentiationof stem cell Invitro.Methods:TheC3H10T1/2stem cell were transfected with 2.67 ml/L pAdEasy-BMP9 Ad-GFP plasmid for 1 week and 2 weeks .The specific transcription factors of cardiomyocytes were detected in stem cells during differentiation with plasmid transfection by RT-PCR and the specific proteins in cardiomy-ocytes were detected by laser confocal microsopy after 1 week;the change of cells ultramicro structure were detected by electron micro-scope after 2 weeks.Results:Cells volume are increscent obviously,cells trend become unanimous,the connection between cells are compact,refractivity of cells enhance conspicuously after induction for 1 week.The expression of NKx 2.5,GATA-4,MEF2C could be de-tected in C3H10T1/2 stem cells with pAdEasy-BMP9 Ad-GFP plasmid tranfection.Cx43 and cTnT can be detected with plasmid tranfec-tion.Cardiomyocytes ultramicro structure can be detected after induction for 2 weeks.Conclusion:BMP9 makes a very important influ-ence in C3H10T1/2 stem cells targeted differentiation into cardiomyocytes。