Effects of granulocyte-macrophage colony-stimulating factor on the expression of cysteine-cysteine chemokine receptor 2 in THP-1 monocytes
- VernacularTitle:粒-巨细胞集落刺激因子对单核细胞表达CCR2的影响
- Author:
Jiong LI
- Publication Type:Journal Article
- Keywords:
Granulocyte-macrophage colony-stimulating factor;
Monocytes;
Cysteine-cysteine chemokine receptor 2
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of granulocyte-macrophage colony-stimulating factor on expressions of CC chemokine receptor 2(CCR2) mRNA and protein in cultured human THP-1 cell lines.Methods:THP-1 cell was stimulated by GM-CSF of different concentrations and time intervals.The mRNA expression of CCR2 was tested by RT-PCR,The protein expression of CCR2 was analyzed by Western blotting.Results:RT-PCR and Western blotting showed that THP-1 cell in control group expressed a low-level of CCR2 mRNA and protein.After stimulated by GM-CSF,the expression of CCR2 mRNA and protein increased in a concentration-and time-dependent manner.Conclusion:GM-CSF could stimulate THP-1 cell to express CCR2 mRNA and protein,in an concentration-and time-dependent manner.
