Simultaneous determination of serum tauro-conjugated bile acids in intrahepatic cholestasis of pregnancy patients by high performance liquid chromatography-tendem mass spectrometry
- VernacularTitle:高效液相色谱-串联质谱法测定妊娠肝内胆汁淤积患者血清牛磺结合型胆汁酸
- Author:
Wenping DENG
- Publication Type:Journal Article
- Keywords:
Bile acids;
Serum;
ICP;
HPLC;
MS/MS
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the individual serum tauro-conjugated bile acid concentration in patients with Intrahepatic Cholestasis of Pregnancy(ICP) by high performance liquid chromatography-tendem mass spectro-metry(HPLC-MS/MS).Methods:Bile acids were extracted from serum samples of ICP,ICP with Diabetes Mellitus,ICP with Virus Hepatitis patients and normal pregnant women.Serum tauro-conjugated bile acids were measured by HPLC-MS/MS.Results:The serum levels of taurocholic acid(TCA),taurochenode-oxycholic acid(TCDCA) and taurodeoxycholic acid(TDCA) in the three groups of ICP,ICP with Diabetes Mellitus,and ICP with Virus Hepatitis were significantly higher than those of the control group(P
