Effect of curcumin on the activity of ATPase in U-251 cells
- VernacularTitle:姜黄素对胶质瘤细胞ATP酶的影响
- Author:
Song CHEN
- Publication Type:Journal Article
- Keywords:
Curcumin;
Human glioma cell U-251;
ATPase;
Apoptosis
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of curcumin on the activity of ATPase and the mechanism of apoptosis in U-251 cell.Methods:U-251 cells were treated with 20,40,80,100?mol/L curcumin for 24 h and the growth inhibition rates of U-251 cells were measured with MTT method.Cell apoptosis was inspected with flow cytometry(FCM).The activities of ATPase were determined by colorimetry method.Results:Curcumin inhibited the proliferation of U-251 cells and induced apoptosis of U-251 cells.level of ATPase in U-251 plasma Membrane was low remarkably.Conclusion:Curcumin induced apoptosis and inhibited proliferation of U-251 cell via inhibition of activation of plasma Membrane ATPase.
