Biological effect of maspin gene on VEGF in hepatocarcinoma cell MHCC-97
- VernacularTitle:抑癌基因maspin在肝癌细胞株MHCC-97中对VEGF的生物学作用
- Author:
Xiong YAN
- Publication Type:Journal Article
- Keywords:
Maspin;
VEGF;
HepatocarcinomacellMHCC-97
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the biological effect of maspin on VEGF(vascular endothelial growth factor,VEGF)in hepatocarcinoma cell MHCC-97 and discuss its significance.Methods:Maspin/pEFIRES-N expressing plasmid was constructed .The changes of maspin and VEGF in the levels of mRNA and protein in hepatocarcinoma cell MHCC-97 before and after the transfection and recombination were detected by RT-PCR and Western blotting.Results:The maspin/pEFIRES-N plasmid was constructed successfully,and was stably transferred into MHCC-97 cell.The expressions of maspin mRNA and protein increased in hepatocarcinoma cell MHCC-97 after the transfection of maspin/pEFIRES-N plasmid,while the expressions of VEGF mRNA and protein decreased ,as compared with those after the transfection of pEFIRES-N plasmid(P
