Study on targetability of lactosaminated matrine liposomes to liver in vivo and inhibition to HepG2 cells in vitro
- VernacularTitle:乳糖脂修饰苦参碱脂质体的肝靶向性和体外抑瘤作用研究
- Author:
Fangyu ZHANG
- Publication Type:Journal Article
- Keywords:
Lactosylphosphatidylethanolamine(Lac-PE);
Matrine;
Liposome;
Liver targeting;
Hepatic carcinoma cell line HepG2
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To prepare lactosaminated matrine liposomes and to study its targetability to liver in mice and efficacy of anti-tumor activities on human hepatoma cell line HepG2 in vitro.Methods:The matrine liposomes were prepared using the reverse-phase evaporation-ultrasonic technique.Lactosylphosphatidy-lethanolamine(Lac-PE) was synthesized and used for modified matrine liposomes..the diameter and entrapment efficiency of it were determined.The RP-HPLC was used for the determination of matrine concentration in mice tissue.The cytotoxic effect of LML on human hepatoma cell line HepG2 in vitro was detected by thiazolyl blue(MTT)assay.Results:The LML were nice and uniform,the particle diameter was between 80 and 150 nm and envelopment rats was 48.1%.Compared with matrine solution,ML and LML exhibited long circulation time.Tissue distribution results proved that the area under curve of liver was significantly difference among modified matrine liposomes,regular matrine liposomes and matrine solutions(P
