Chemical composition of Cliffbean polysaccharide
- VernacularTitle:玉郎伞多糖化学成分的研究
- Author:
Xing LIN
;
Weizhe JIANG
;
Yang JIAO
;
Shijun ZHANG
;
Renbin HUANG
- Publication Type:Journal Article
- Keywords:
Cliffbean;
polysaccharide;
isolation;
purification;
HPGPC
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the isolation and composition of Cliffbean(Millettia pulchra kurz)polysaccharide.METHODS:Cliffbean was extracted by boiling water.The polysaccharide in the filtrate was precipitated fractionally by alcohol.The protein in the product was removed by Sevag method,and was further purified by DEAE ion-exchange cellulose(DEAE-52).The component of the saccharide was determined by GC and TLC.RESULTS:The polysaccharide obtained in this way didn't contain protein and nucleic acid.It showed a single polysaccharide when identified by means of Sephadex G-75 column or High Performance Gel Permeation Chromatography(HPGPC).It was composed of glucose and arabinose.CONCLUSION:Cliffbean polysaccharide was first isolated from its root and was one of the important components.
