Macrophage mannose receiver model and its application on screening of compound prescription of traditional Chinese herbs
- VernacularTitle:巨噬细胞甘露糖受体模型的初步建立及其在中药复方筛选中的应用
- Author:
Weiyi TIAN
;
Dengxiang DONG
;
Juan YANG
;
Ping WANG
;
Wenjia WANG
- Publication Type:Journal Article
- Keywords:
macrophages;
mannose receiver;
traditional Chinese medicine;
screening model
- From:
Chinese Traditional Patent Medicine
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish macrophage mannose receiver Model(MMR),and use it to screen active component with mannose receiver(MR)as target from traditional Chinese herbs.METHODS:The mouse abdominal macrophages was hatched with D-mannose and D-galactose of the different concentration,and the flow cytometry and fluorescence microscope were used to measure the antagonistic effect of M-FITC-BSA(Mannose-fluorescein isothiocyanate-bovine serum albumin)with D-mannose and D-galactose.After the MMR screening model was established to screen MR union ingredients of polysaccharide ingredients from six compound prescriptions,such as Siwu Decoction and so on.RESULTS:Both of measuring methods showed that when D-mannose concentration increased the relevance ratio of M? marked with M-FITC-BSA decreases(P
