Study on Extraction Process of Polysaccharides from Elaeagnus angustifolia L. by Enzymatic- assisted Ultrasonic Methods
- VernacularTitle:复合酶辅助超声波提取沙枣多糖的工艺研究
- Author:
Yijun LIAN
;
Bingqi LI
;
Hong LIU
- Publication Type:Journal Article
- Keywords:
Elaeagnus angustifolia L.;
polysaccharides;
enzyme;
ultrasonic;
extraction process
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the technological conditions of polysaccharide extraction from Elaeagnus angustifolia L. by enzymatic-assisted ultrasonic. Methods The effects of different enzymes, enzymolysis temperature, enzyme dosage, enzymolysis time, material-water ratio, crushing degree, ultrasonic time and ultrasonic temperature on the extraction rate of Elaeagnus angustifolia L. polysaccharide was analyzed. Results The optimal technological condition was as follows:1.5% cellulase, 1.5% pectic enzyme, enzymolysis time 40 min, enzymolysis temperature 55 ℃, ultrasonic time 25 min, ultrasonic power 400 W, material- water ratio 1∶30, and temperature 60 ℃. In these conditions, the extraction efficiency of polysaccharide was 12.35%. Conclusion The process is simple and practicable, and can be used for the extraction of Elaeagnus angustifolia L. polysaccharide.