Content Determination of 3-Epibetulinic Acid in Nodus Nelumbinis Rhizomatis Charcoal
- VernacularTitle:藕节炭中3-表白桦脂酸含量的测定
- Author:
Shanxin LIU
;
Meng WANG
;
Guangqian JIN
- Publication Type:Journal Article
- Keywords:
Nodus Nelumbinis Rhizomatis Charcoal;
3-epibetulinic acid;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the content determination of 3-epibetulinic acid in Nodus Nelumbinis Rhizomatis Charcoal. Methods HPLC was used on Lichrospher C18 column (4.6 mm?150 mm, 5 ?m) with mobile phase of acetonitrile-0.2% glacial acetic acid (75∶25), the flow rate of 1 mL/min, the column temperature of 30 ℃ and the detection wavelength of 207 nm. Results 3-epibetulinic acid has a good linearity within 0.365 4～3.654 0 ?g (r=0.999 6) and the average recovery was 98.83% with RSD=2.47%. The content of 3-epibetulinic acid in Nodus Nelumbinis Rhizomatis Charcoal from different region varied from 0.048 3% to 0.575 8%. Conclusion This method is simple, accurate, with good reproducibility, and can be used for the content determination of 3-epibetulinic acid in Nodus Nelumbinis Rhizomatis Charcoal.
