Determination of Hesperidin in Henggu Gushangyu Tablets by HPLC
- VernacularTitle:HPLC法测定恒古骨伤愈片中橙皮苷的含量
- Author:
Dajun ZHANG
;
Zhaohua WANG
- Publication Type:Journal Article
- Keywords:
Henggu Gushangyu Tablets;
Hesperidin;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of Hesperidin in Henggu Gushangyu Tablets by high performance liquid chromatography. Methods The determination was conducted on Shim-pack-C18 column (150 mm?4.6 mm, 5 ?m) with mobile phase of acetonitrile-0.4% phosphoric acid water (20∶80). The detection wavelength was 283 nm. Results Good linear relationship of Hesperidin between area and amount was noted for 0.194～0.970 ?g, with a correlation coefficient of 0.999 9. The average recovery was 98.50%, and RSD was 0.63%. Conclusion The established method is simple, accurate and sensitive, and can be applied to the quality control of the preparation.
