Experimental Study of Kidney Aquaporin-2 Expression in Lung-Qi Deficiency Model Rats
- VernacularTitle:肺气虚证模型大鼠肾组织水通道蛋白2表达的变化
- Author:
Zhe WANG
;
Hongwei SUN
;
Hao WANG
- Publication Type:Journal Article
- Keywords:
Lung-Qi deficiency;
kidney tissues;
aquaporin-2;
rat
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of kidney aquaporin-2 (AQP2) expression in Lung-Qi deficiency model rats and provide experimental evidence for the traditional Chinese medical theory that the kidney is related with the lung in charge of regulating the water passage. Method Twenty healthy rats were divided into the model group and the control group. The method of immunohistochemistry and Western blot was used to determine the expression of AQP2 in kidney. RT-PCR was applied to analyze the expression of AQP2 mRNA in lung and kidney tissues. Result The expression of AQP2 and AQP2 mRNA in kidney in model group was higher than in the control group. Conclusion The expression of AQP2 in kidney was higher in Lung-Qi deficiency model rats. One of the substance basis of "lung in charge of regulating the water passage" may be related to the change of AQP2 expression in the kidney tissues.
