Effect of Qingdushuan on PTEN-MDM2-p53 Network of Cervical Cancer SiHa Cell
- VernacularTitle:清毒栓对宫颈癌SiHa细胞及PTEN-MDM2-p53网络环路的影响
- Author:
Yanyan YU
;
Zhe JIN
;
Jiaoying LOU
- Publication Type:Journal Article
- Keywords:
Qingdushuan;
Cervical cancer;
SiHa cells;
PTEN-MDM2-p53 network
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effect of Qingdushuan on SiHa cells of cervical cancer proliferation and protein expression in PTEN-MDM2-p53 network from the level of cell biology and molecular biology. Methods SiHa cells were cultivated with 4% containing serum. Normal control group, blank serum group, Qingdushuan group, Baofukang group and interferon group were established. The influence of inhibiting and proliferating SiHa cell was detected by MTT assay. Three protein expression was detected by Western blot method. Results After cultured with drug containing serum, the number of cells decreased, 4% concentration of serum for 72 h was the strongest. Compared with the control group, there were significant differences (P
