Optimization of extracting technology for flavonoids in Selaginella tamariscina with orthogonal design
- VernacularTitle:正交设计优选卷柏中总黄酮的提取工艺研究
- Author:
Weisheng FENG
;
Xianmin ZHAO
;
Yanzhi WANG
;
Yue WEI
;
Xiaoke ZHENG
- Publication Type:Journal Article
- Keywords:
Selaginella amariscina(Beauv.) Sping;
total flavonoids;
orthogonal design
- From:
Chinese Traditional and Herbal Drugs
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extraction technology for total flavonoids in Selaginella tamariscina.Methods To determine the content of total flavonoids by UV and the content of index constituent of amentoflavone by HPLC.The optimum extraction condition was investigated by orthogonal design and the extraction quantity was regarded as the investigated index.Results The optimum extracting condition was A1B2C2D2 with the extraction quantity of total flavonoids as the investigated index.The optimum extracting condition was A1～3B1～3C2D2 with the extraction quantity of amentoflavone as the investigated index.The optimum extracting condition was A1B2C2D2.That is adding ten times amount of 95% alcohol and refluxing twice,2 h once.Conclusion The optimum technology is stable and feasible for the extraction of S.tamariscina.
