Effect of ATP50 on GH-induced testosterone secretion in isolated rat Leydig cells
- VernacularTitle:ATP50对生长激素诱导大鼠睾丸间质细胞睾酮分泌的影响
- Author:
Bo PENG
- Publication Type:Journal Article
- Keywords:
ATP50;
Leydig cells;
Anti-oligodeoxynucletides(anti-ODN);
Testosterone
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Toinvestigate the effect of ATP50 on GH-induced testosterone secretion in isolated SD rat Leydigcells.Methods:The Leydig cells were treated with ATP50 antisense oligodeoxynucletides(Anti-ODN)in GH group,nonsense tat ODN in control group,cAMP and testosterone in culture medium was detected by radioimmunoassay,expression of ATP50 protein was investigated by immunohistochemistry.Results:Antisense ATP50 ODN significantly inhibited GH-induced testosterone secretion of isolated rat Leydig cells in a dose-dependent manner(P
