Identification and analysis of membrane estrogen receptor in inters titial cells of Cajal
- VernacularTitle:小鼠肠道Cajal间质细胞膜雌激素受体的检测和分析
- Author:
Fan LI
- Publication Type:Journal Article
- Keywords:
Estrogen;
Membrane estrogen receptor;
Interstitial cells of Cajal
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect and analyze the membrane estrogen receptor(mER)in primary cultured interstitial cells of Cajal(ICC).Methods:Interstitial Cells of Cajal(ICC)'s surface binding sites for 17-?estradiol(E2)were detected by cell-impermeant ligand using confocal microscopy.Radioligand binding assay and Scatchard software were used to analyzed the characteristics of mER.Results:Immunofluorescence shows the staining pattern of nonfixed,nonpermeabilized ICC incubated with E2BSAFITC.The radioligand binding assay were analyzed by Scatchard software,The Bmax of mER was 45.75 fmol/mg protein and the kD was 0.717 3 nmol/L.Conclusion:A form of the estrogen receptor is present within the cell membrane of ICC and maybe capable of mediating rapid effect of estrogen.
