Improvement of primary culture of cardiac myocytes in neonatal rat
- VernacularTitle:新生大鼠心肌细胞原代培养方法的改良
- Author:
Jianan WANG
- Publication Type:Journal Article
- Keywords:
Cardiac myocyte;
Primary Culture;
Neonatal rat
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss how the more convenient primary culture method of rats'cardiac myocytes to increase the quantity,livability and purity of isolated cells.Methods:Neonatal rats'cardiac myocytes were isolated by co-digestion of trypsin and collagenase Ⅱ,and then cultured in vitro.Morphocytology changes were observed and cell purity was evaluated by immunofluorescence.Results:Isolation of cardiomyocytes was with a good quantity and high livability.Cells adhered and impulsed well.Immunofluorescence staining confirmed the cell with purity higher than 95%.Conclusion:The modified cell culture method can obtain the Cardiomyocytes with good growth state.
