The clone of OPG and its effects on the differentiation of bome morrow stem cells
- VernacularTitle:骨保护素的克隆及对骨髓间充质干细胞分化的影响
- Author:
Fu CHEN
- Publication Type:Journal Article
- Keywords:
Adenovirus;
Human OPG;
Bone marrow stem cells
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Toclone the OPGgene ofhuman beingand construct its recombinant adenovirus and then observe its effects on the differentiation of bone marrow stem cells(BMSCs).Methods:The cDNAs of human OPG in hepatic tissue were obtained by using RT-PCR method.The cDNAs then were recombined with competent homologous cells to form the AdOPG and the shuttle vector was used during this stage.The rabbit BMSCs were isolated and cultured in vitro,and were infected with AdOPG.The OPG expression of BMSCs was identified by RT-PCR and Western blot.The expression of ALP was detected by chromatometry and ALP dyeing five days after the infection.Results:The OPG gene of human being was successfully cloned and its sequence consistent with the Gene bank.The titre of constructed AdOPG can reach 109 efu/ml.The ALP activity of BMSCs was(21 024?507)IU five days after the infection of AdOPG,whereas the value was(3 079?89)IU in the control group and(2 156?78)IU in the blank control group.Conclusion:The OPG can induce the osetogeinc differentiation of BMSCs.
