Construction of the standard curve for Real-time PCR detecting the expression of Angptl3 in rats
- VernacularTitle:实时荧光定量PCR检测大鼠组织Angptl3表达的标准曲线构建
- Author:
Jihong WANG
- Publication Type:Journal Article
- Keywords:
Real-time PCR;
Angptl3;
Standard curve;
SD rat
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct the standard curve for real-time PCR detecting the tissue expression level of Angptl3 in SD rat.Methods:The total RNA of the SD rat's liver was the template.We got lots of the Angptl3 partial DNA sequence by RT-PCR.The products were cloned into the pUCm-T vector,Then we diluted the solution of plasmid and used real-time PCR(SYBR GreenⅠ)to construct the standard curve.Results:The correlation coefficient of the standard curve was 0.997 6.The reaction efficiency was 0.862 3.The correlation and the amplify efficiency were good.Conclusion:It is successful to construct the standard curve for real-time PCR detecting the tissue expression level of Angptl3 in SD rat.
