Effect of silencing FAK gene expression by siRNA on characteristics of human breast cancer MCF-7 cell line
- VernacularTitle:siRNA沉默FAK基因表达对乳腺癌MCF-7细胞生物学特性的影响
- Author:
Jie MA
- Publication Type:Journal Article
- Keywords:
RNA interference;
Breast cancer;
Focal adhesion kinase;
Proliferation;
Tumor invasion
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect ofRNA interferenc(RNA)ion FAK gene expression and the biological characteristics of human breast cancer MCF-7.Methods:The constructed recombinant plasmid FAK-siRNA was transfected into human breast cancer MCF-7 cell using LipofectamineTM 2000.The transfection efficiency was observed under fluorescence confocal microscope.Expression of FAK mRNA and protein were detected by reverse transcription polymerase chain reaction(RT-PCR)and Western blot.Distribution of cell cycle was assessed by flow cytometry.Cell penetrate matrigel capacity were determined by invitro experiment.Results:FAK-siRNA effectively inhibited FAK mRNA and protein expression(P
