Zileuton,a 5-LOX inhibitor,inhibits proliferation and induces apoptos is of SGC-7901 gastric cancer cells

VernacularTitle:齐留通对胃癌SGC-7901细胞生长的影响
Author: Min PAN
Publication Type:Journal Article
Keywords: Zileuton; Gastric cancer; SGC-7901 cells; 5-LOX inhibitor; Apoptosis
From: Journal of Chongqing Medical University 2003;0(05):-
CountryChina
Language:Chinese
Abstract: Objective:To evaluate the efficacy of 5-LOX inhibitor zileuton on the proliferation and apoptosis of human gastric carcinoma cell line SGC-7901,and to explore the mechanisms of apoptosis induced by zileuton.Methods:The proliferation of SGC-7901 cells was detected by thiazolyl blue tetrazolium bromide(MTT)assays.TUNEL staining was used to evaluate the apoptosis of SGC-7901 cells.The changes of Bax,Bcl-2 and caspase-3 mRNA expression were studied by reverse transcription polymerase chain reaction.Bax,Bcl-2 and caspase-3 protein expression were analyzed by immunocytochemistry in SGC-7901 cells treated with zileuton.Results:Zileuton can decrease the proliferation significantly in a dose-dependent and time-dependent manner in SGC-7901 cells.TUNEL staining showed that the apoptosis rate was elevated with the increase of zileuton concentration(P