Enhancement of cytotoxic effect of epirubicin on gastric carcinoma cell line SGC-7901 by survivin antisense RNA and signaling pathway
- VernacularTitle:生存素反义寡核苷酸在胃癌化疗中对表阿霉素的增敏性及其信号转导途径研究
- Author:
Jun HOU
- Publication Type:Journal Article
- Keywords:
Antisense RNA;
Survivin;
Epirubicin;
Signaling pathway
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether interfering survivin has the potential to sensitize stomach cancer cells to Epirubicin.Methods:By the method of liposome transfection,survivin antisense oligodeoxynucleotide was transfected into human stomach cancer SGC-7901 cell line.Treat cells with different concentrations of survivin ASODN and Epirubicin respectively to select the best combination trough assessing the growth of cells by MTT assay.Cells were randomly divided into control group,ASODN group,Epirubicin and combination group.Western blot analysis was used to check the survivin protein expression;inhibitive rate were detected by MTT assay;Flow cytometric analysis was used to detect the cycle status of SGC-7901 cells.The apoptosis was screened according to the result of Annexin V-FITC.Reverse transcription polymerase chain reaction(RT-PCR)were applied to detect the expression of bcl-2、SMac mRNA.Results:MTT assay showed the proliferation of SGC-7901 cells was retarded obviously contrasting to control group.The level of survivin protein expression in ASODN group are lower than control group according to Western blot analysis.Annexin V-FITC show that the apoptosis rate of ASODN group cells increases.The level of SMac mRNA expression in ASODN group are higher than control group according to RT-PCR analysis.Conclusion:Survivin siRNA can sensitize stomach cancer cells to Epirubicin.Up-regulating the SMac expression might be one of mechanisms.
