The study of disseminated tumor cell(DTC)and p53 gene in mouse model of laryngocarcinoma
- VernacularTitle:喉癌动物模型中播散肿瘤细胞与突变型p53的研究
- Author:
Wenqi ZUO
- Publication Type:Journal Article
- Keywords:
Laryngocarcinoma;
Nude mouse;
Disseminated tumor cell;
p53 gene;
Tumour metastasis and prognosis
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:We established nude mouse model of laryngocarcinoma and detect the disseminated tumor cell of peripheral blood and mutant p53 in tumour tissue,to research the correlation of DTC with variant p53 gene and their value of predicting cancer metastasis and prognosis in laryngocarcinoma.Methods:There are 40 nude mice in the experimental group which accepted hypodermic injection of laryngocarcinoma cell,10 in control.By the reverse transcription polymerase chain reaction and nested polymerase chain reaction(Nest-PCR),we tested CK19mRNA in nude mouse's peripheral blood,as a marker of disseminated cancer cell at the 10th weeks after tumor-planting.We also tested the expression of p53 gene in tumour tissue by RT-PCR and immunohistology.After the nude dead,we dissect lung,liver and lymph node and use continuous pathological dyeing confirmed whether the transfer.Results:The positive rate of DTC was 50.0%(20/40),but negative in the control group,the difference was significant(P
