Immunoregulation of the Total Polysaccharide from Sijunzi Decoction on Mice Intestinal Intraepithelial Lymphocytes
- VernacularTitle:四君子汤复方总多糖对小鼠肠上皮间淋巴细胞的免疫调节作用
- Author:
Dapeng ZHANG
;
Lian ZHOU
;
Zhimin ZHANG
;
Qing WANG
;
Peixun WANG
- Publication Type:Journal Article
- Keywords:
Total polysaccharides from Sijunzi Decoction;
Intestinal intraepithelial lymphocytes;
CD3;
CD69;
IFN-?;
IL-2
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To imestigate the effect of total polysaccharides(TP) from Sijunzi Decoction(SD) on the immune function of mice intestinal intraepithelial lymphocytes(iIEL).Methods NIH mice were eaqually randomized to 4 groups:control group,model group,TP-SD group,free-protein polysaccharides from SD(FPP-SD) group.Mice iIEL was extracted by discontinuous density method.The percentage of CD3+ and CD69+ in iIEL were detected by flow cytometer.IL-2 and IFN-? expression in iIEL supernatant was detected by ELISA.Results The percentage of CD3+ lymphocyte in iIEL was 88.46 %.The percentage of CD69+ lymphocyte and the levels of IL-2 and IFN-? in iIEL were significantly decreased in the model group compared with those in the normal control group(P
