Study on the HPLC Characteristics of Fructus Schisandrae Chinensis Ertract by Low-temperature Water-extract Method
- VernacularTitle:五味子低温水提取物HPLC特征图谱的研究
- Author:
Huiling WEN
;
Mansha LI
;
Wenyao SONG
- Publication Type:Journal Article
- Keywords:
Fructus Schisandrae Chinensis;
Low-temperature extraction;
schizandrol A;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC determination method for the effective constituents extracted from Fructus Schisandrae Chinensis by low-temperature water extraction.Methods The assay was conducted on Kromasil C18 column(250 mm? 4.6 mm,5 ? m)using a gradient elution with acetonitrile-water as mobile phase.The flow rate was set at 1.0 mL/min,column temperature was kept at 30 ℃,and the detection wavelength was at 240 nm.Results The linear range for schizandrol A was 5.0~ 200.0 ? g/mL(r=0.999 9),and the average recovery was 101.27 % with RSD being 1.97 %(n=6).Conclusion The method is simple,reliable and reproducible for the determination of schizandrol A.It can be used for quality control of preparations including Fructus Schisandrae Chinensis.