Determination of Prim-O-glucosylcimifugin in Biyan Tablets by HPLC
- VernacularTitle:高效液相色谱法测定鼻炎片中升麻素苷含量
- Author:
Qiaojun HE
;
Youheng GAO
;
Zhixiong WEI
- Publication Type:Journal Article
- Keywords:
Biyan Tablets;
Prim-O-glucosylcimifugin;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the determination of prim-O-glucosylcimifugin in Biyan Tablets by HPLC.Methods The HPLC determination was carried out on Diamonsil C18 column(4.6 mm? 250 mm,5 ? m).The mobile phase consisted of methanol-acetonirile-H2O(18 :12 :70) with a flow rate of 1.0 mL/min,and the ultraviolet detection wavelength was set at 254 nm.Results Prim-O-glucosylcimifugin showed a good linearity in the range of 0.1888～ 1.534 ? g with the peak area score,the coefficient was 0.9997,and the average recovery was 101.74 %(RSD=2.97 %).Conclusion The method is accurate and reliable,and can be used for the quality control of Biyan Tablets.
