Determination of Piperine in Root of Piper nigrum L.by HPLC
- VernacularTitle:HPLC法测定胡椒根中胡椒碱的含量
- Author:
Jiamin HU
;
Shaozhong PENG
;
Huifang ZENG
;
Jiannan CHEN
;
Ziren SU
;
Xiaoping LAI
- Publication Type:Journal Article
- Keywords:
Root of Piper nigrum L.;
Piperine;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a RP-HPLC method for the determination of piperine in the root of Piper nigrum L.Methods RP-HPLC was carried out on Luna C18 column(250 mm? 4.60 mm,5 ? m) with the column temperature of 35 ℃.The mobile phase consisted of a mixture of methanol-water(77 :23) at a flow rate of 1.0 mL? min-1.The determination wavelength was at 343 nm.Results The calibration curve was linear within the concentration range of 0.164 ? g～ 0.984 ? g,r=0.9996,and the average recovery was 98.09 %,RSD=2.67 %(n=9).The average content of piperine in three batches of pepper roots was in the range of 6.67～6.77mg?g-1.Conclusion Pepper root contains piperine,and this method is suitable for the quality control of the root of Piper nigrum L.
