Determination of Baicalin content in Pudilan Xiaoyan Capsule by HPLC
- VernacularTitle:高效液相色谱法测定蒲地蓝消炎胶囊中黄芩苷含量
- Author:
Zesheng LIN
- Publication Type:Journal Article
- Keywords:
Pudilan Xiaoyan Capsule;
Baicalin;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quality standard for Pudilan Xiaoyan Capsule(PXC).Methods The content of baicalin in PXC was determined by HPLC.The AichromBond-ODS-C18 column(4.6 mm? 150 mm,5 ? m) was used with column temperature being at 30 ℃ and injection volume being 10 ? L.The mobile phase was consisted of CH3OH-H20-H3PO4(40 :60 :0.2) with the flow rate being 1.0 mL/min.The detection wavelength was at 280 nm.Results A good linearity was obtained in the range of 0.062～ 0.372 ? g of baicalin with a good correlation coefficient(r=0.9998).Conclusion The method is accurate and good reproducibility.It can be used effectively for the quality control of Pudilan Xiaoyan Capsule.
