HPLC Content Determination of Luteolin in Callicarpa nudiflora Hook.et Arn.
- VernacularTitle:HPLC法测定裸花紫珠药材中木犀草素的含量
- Author:
Rong HU
;
Min YAO
;
Yuyun LI
;
Caiqing ZHU
- Publication Type:Journal Article
- Keywords:
Callicarpa nudiflora Hook.et Arn;
Luteolin;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of luteolin in Callicarpa nudiflora Hook.et Arn..Methods HPLC was performed with chromatographic conditions as follows:Alltima C18 column(250 mm? 4.6 mm,5 ? m)with column temperature at 25 ℃,the mobile phase of methanol-water-phosphate(55:45:0.4),flow rate at 1 mL/min,and the detection wavelength at 348 nm.Results The linearity of luteolin was good in the range of 0.032 46～ 0.129 84 ? g(r=0.999 5).The average recovery was 99.52 % and RSD was 1.58 %.Conclusion The method is simple,feasible and reproducible,and can be used to control the quality of Callicarpa nudiflora Hook.et Arn..
