Effects of Sancaoanqian Decoction on Expression of IL-1?, IL-6 and TNF RⅡ in Experimental Autoimmune Prostatitis Rats
- VernacularTitle:三草安前汤对慢性非细菌性前列腺炎模型大鼠IL-1?、IL-6及TNFRⅡ表达的影响
- Author:
Qing ZHOU
;
Wei XIONG
;
Guomin ZHANG
- Publication Type:Journal Article
- Keywords:
Sancaoanqian decoction;
chronic autoimmune prostatitis;
cytokine;
animal model
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Sancaoanqian decoction on expression of cytokine IL-1?, IL-6 and TNF RⅡ in experimental autoimmune prostatitis rats, and explore the target protein of cytokine of Chinese herbal compound in treating chronic nonbacterial prostatitis. Methods Seventy-two male Wistar rats were divided randomly into six groups and 12 rats in each group. All groups were made experimental autoimmune prostatitis rat model by injecting SC purified prostate protein with FCA except normal group. The normal and model group were given saline, western medicine group was given indomethacin, large, medium and small doses decoction groups were treated with large, medium and small doses of Sancaoanqian decoction. All rats were sacrificed after 30 days. The mRNA and protein expression of cytokines were tested by methods of immunohistochemistry and fluorescent quantitation RT-PCR. Results Sancaoanqian decoction could reduce the mRNA and protein expression of IL-1?, IL-6 in experimental autoimmune prostatitis rats. There were no significant difference between large and medium doses decoction group, but had a better activity than small dose. The expression of TNF RⅡ were reduced in all doses groups and no difference among three doses of Sancaoanqian decoction. Conclusion Sancaoanqian decoction can regulate the immune function in chronic prostatitis rat model. Down-regulation of cytokines maybe one of important mechanisms in treating chronic prostatitis.
