Effects of Tripterygium hypoglaucum(Level) Hutch Extract on T Lymphocyte Subpopulations in Mice with Graft-versus-host Disease
- VernacularTitle:昆明山海棠提取物对移植物抗宿主病小鼠T细胞亚群的影响
- Author:
Sheng LI
;
Kailin XU
;
Zhenyu LI
;
Xiuying PAN
- Publication Type:Journal Article
- Keywords:
TRIPTERYGIUM HYPOGLAUCUM (LEVEL)HUTCH/pharmacology;
GRAFT-VERSUS-HOST DISEASE/TCD therapy;
T-LYMPHOCYTES SUBSETS/blood;
SKIN/pathology;
LIVER/pathology;
SMALL INTESTINE/pathology;
DISEASE MODELS,ANIMAL;
MICE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the preventive effects of Tripterygium hypoglaucum(Level)Hutch(THH)extract on T lymphocyte subpopulations in mice with graft-versus-host disease(GVHD)and to explore its protective mechanism.Methods 2?107 bone marrow cells mixed with 2?107 spleen cells from C57BL/6 mouse were transplanted into the myeloablative irradiated inbred BALB/c mouse.The experiment groups were designed as follows:model control group,cyclosporine A(CsA,10 mg?kg-1?d-1)group,THH(400 mg?kg-1?d-1)group,combination group(CsA 5 mg?kg-1?d-1 + THH 100 mg?kg-1?d-1).The ocurrence of GVHD was assessed by signs of weight loss,diarrhea,ruffled fur,hunched posture and histologic changes of skin,liver and small intestines.Chimerism was detected by monitoring the H-2b molecule in bone marrow cells of recipient mice with flow cytometer.The percentages of CD+3CD+4 and CD+3CD+8 T cells in peripheral blood were detected by flow cytometer.Results The survival time of CsA group,THH group and combination group were prolonged as compared with that of the model control group(P
