Experimental Study of Intervention of Yiqi Huaxian Decoction on Rats Pulmonary Fibrosis
- VernacularTitle:中药益气化纤汤抗大鼠肺纤维化的实验研究
- Author:
Wangxi CAI
;
Shanshan YIN
;
Jianfang CHANG
- Publication Type:Journal Article
- Keywords:
YIQI HUAXIAN DECOCTION/pharmacology;
PULMONARY FIBROSIS/TCD therapy;
TRANSFORMING GROWTH FACTOR ?1;
ALVEOLAR MACROPHAGES/pathology;
INTERSTITIAL MACROPHAGES/pathology;
DISEASE MODELS,ANIMAL;
RATS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Yiqi Huaxian Decoction (YHD) on transforming growth factor ?1(TGF-?1 )mRNA expression in rats alveolar macrophages (AM) and pulmonary interstitial macrophages (IM).Methods Ninety-six Wistar rats were randomized into sham-operation group,model group,YHD (4 g?kg-1?d-1) group,and hydrocortisone (20 mg?kg-1?d-1) group.Bleomycin A5 was injected into the rats' trachea to induce pulmonary fibrosis.AM and IM were isolated for the culturing after modeling for 7 and 28 days.Molecular in-situ hybridization (ISH) was used for the detection of TGF-?1 expression in AM and IM.Results Percentage of cells in AM and IM with TGF-?1 positive expression and the score of optic density were increased in the model group (P
