Study on Effective Polysaccharide of Anti-Human Lung Cancer A549 Cells in Zhou’s Prescription for Resolving Mass
- VernacularTitle:周氏克金岩方抗A549肺癌细胞多糖组分的研究
- Author:
Meiping YANG
;
Lian YIN
;
Xu ZHANG
- Publication Type:Journal Article
- Keywords:
Zhou’s Prescription;
polysaccharides;
anti-lung cancer of A549
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study content and ascription of the composition of the polysaccharides from Zhou’s Prescription for Resolving Mass. Method The polysaccharide was extracted by ethanol precipitation, and purified by savage method. DEAE-cellulose anion exchange resin was used to prepare the composition of polysaccharides. UV-spectra was used to detect protein and IR-spectra was used to analyses the ascription of the composition of polysaccharides. The content of the composition of polysaccharides was determined by anthranone-sulfuric acid method. Result There was no absorption at 240 nm and 260 nm. The IR spectrum graph of the polysaccharides was similar to Polygonatum odoratum, Radix Ranunculi Ternati and Agrimonia pilosa Ledeb. The content of the composition of polysaccharides was 1.41%. Conclusion The composition of polysaccharides does not contain proteins and nucleic acids, and come from Polygonatum odoratum, Radix Ranunculi Ternati and grimonia pilosa Ledeb.
