Preparation of Long-circulating Liposome Containing Etoposide and Its Stability in Rats Plasma
- VernacularTitle:足叶乙苷长循环脂质体的制备及血浆中稳定性研究
- Author:
Jun SHI
;
Yi CHENG
;
Weihong CHEN
;
Yangyang SU
- Publication Type:Journal Article
- Keywords:
ETOPOSIDE/production & preparation;
LONG-CIRCULATING LIPOSOMES/methods;
CHROMATOGRAPHY,HIGH PRESSURE LIQUID
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the preparation of long-circulating liposomes (LCL) containing etoposide and to observe its stability in rats plasma.Methods The etoposide-containing liposome was prepared by ethanol injection method. Polyethylene glycol-distearoyl phosphatidylethanolamine (PEG2000-DSPE) was used to modify the membrane of the liposome. Reversed-phase high pressure liquid chromatography (RP-HPLC) was used to detect the concentration of the liposome,and dynamic release method was used to study its stability in mice plasma.Results The mean size of the LCL containing etoposide was (180?26) nm,and the mean concentration of etoposide was (4.78?0.22) mg/mL,with the entrapment efficiency being (88.71?8.2)%. The leakage ratio of the conventional liposome containing etoposide and LCL containing etoposide in mice plasma were (80.14?1.59)% and (46.72?2.61)%,respectively.Conclusion LCL containing etoposide with high entrapment efficiency and low leakage rate can be obtained by using ethanol injection method. Additionally,modification by PEG2000-DSPE could raise the stability of the liposome.
