Isolation and PC12 cell proliferative protein fraction from pilose antler and its activity
- VernacularTitle:梅花鹿鹿茸中促PC12细胞增殖蛋白的分离和活性研究
- Author:
Lijing KE
;
Yilei NIE
;
Xiuyun YE
;
Yushu HUO
;
Pingfan RAO
;
Jianwu ZHOU
- Publication Type:Journal Article
- Keywords:
pilose antler;
size-exclusive chromatography;
negative ion-exchange chromatography;
PC12 cells;
cell proliferation
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The lyophilized pilose antler water extract(PAE) was isolated,and their cell proliferation on PC12 cells was observed.Methods S-200 Size-exclusive gel and DEAE negative ion-exchange liquid chromatograph were employed to fractionate the PAE.SDS-PAGE was employed to analyze the proteins composition of PAE.The protein concentration was determined by Folin-Phenol assay.The proliferation rates of PC12 cells were measured by MTT assay.Results The proliferation rate of PAE on PC12 cells at 13.3 mg/mL was 47%(P
