A study of drug resistance and related genes of imipenem-resistant pseudomonas aeruginosa
- VernacularTitle:耐亚胺培南铜绿假单胞菌的耐药性及相关基因研究
- Author:
Biao XU
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Imipenem;
Resistance mechanism;
Gene
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanisms of imipenem resistance in Pseudomonas aeruginosa.Methods:Drug resistance of 69 isolates of imipenem-resistant Pseudomonas aeruginosa(IRPA)were tested by Kirby-Bauer disk diffusion method;IMP and VIM genes of metallo-?-lactamases,and outer membrane protein D2(OprD2)gene of IRPA were detected and analyzed by PCR method.Results: All 69 isolates were multidrug resistant,and the sensitivity rates of AMK,CFS and FEP were highe.The positive rates for IMP gene and VIM gene were 2.9%(2/69)and 5.8%(4/69),respectively.46 of 69(66.7%)isolates presented with deletion for OprD2 gene. Conclusion:The drug resistance of IRPA is a serious issue.The deletion mutation of OprD2 gene is one of important mechanisms of imipenem resistance in these tested P.aeruginosa isolates.
