Effect of GS K-3? on the cell cycle and metastasis of human gastric carcinoma cell
- VernacularTitle:糖原合酶激酶3-?对胃癌细胞生长周期和转移的影响
- Author:
Yanli WANG
- Publication Type:Journal Article
- Keywords:
Glycogen synthase kinase-3?;
Gastric carcinoma;
Cell cycle;
E-cadherin
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of GSK-3? on the cell cycle and metastasis of human gastric carcinoma cell.Methods:Influencing the GSK-3? activity of human gastric carcinoma cell line SCG-7901 by LY294002 with or with not LiCl treatment.The expression of total GSK-3? and p-GSK-3?ser9 were detected in protein level by western blotting,cell proliferation and cell cycle distribution were tested by MTT and FCM respectively,the expression of E-cadherin and ?-catenin were measured with S-P immunohistochemistry and the cell-ground substance adhesion was tested by cell adherence assay.Results:The expression of total GSK-3? was constant and the protein level of p-GSK-3? ser9 was down regulated after exposured to LY294002 by 72 h,the cell proliferation was inhibited,cell cycle was arrest at G0/G1 phase,the E-cadherin level was positively regulated,the expression of ?-catenin and the adhesion rate were decreased.The effect on the proliferation、cell cycle、the expression of E-cadherin and ?-catenin and the adhesion by the LY294002 were partially abrogated by lithium chloride.Conclusion:GSK-3?could negatively regulate the proliferation of human gastric carcinoma cell by leading cells to G1-S arrest and inhibit its metastasis by positively regulating the expression of E-cadherin.
