Antitumor and immune regulating effect of exosomes cancer vaccine derived from H_(22) in vivo
- VernacularTitle:H_(22)源exosomes瘤苗在小鼠体内的抗肿瘤及免疫调节作用
- Author:
Shaohua WANG
- Publication Type:Journal Article
- Keywords:
Exosomes;
Hepatocellular carcinoma;
Antitumor immunity;
Cancer vaccine
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence ofexosomes tumor vaccine on immunity and grouth ofmouse hepatocellular carcinoma xenograft in mice.Methods:Hepatoma carcinoma H22 cell-derived exosomes were isolated and purified from the culture supernatants by serial ultracentrifugation and sugar density ultracentrifugation.BALB/c mice inoculated with H22 cell were used as tumor models.The mice inoculated with H22 cell were divided into two groups at random:exosomes group and PBS control group.15 days later,the mice were killed;then the tumor inhibitory rate was calculated.The spleens and thymuses were weighed,and the effect of exosomes on spleens and thymus index was determined.The proliferation and cytotoxicity of splenocytes were detected by MTT assay.The level of IL-2 and INF-? in splenocyte culture supernants was detected by enzyme-linked immunoabsorbent assay(ELISA).Results:Exosomes cancer vaccine depressed the growth of tumor remarkably,and its tumor inhibitory rate was 42.38%.Compared with these in PBS control group,the spleen indexes and thymus indexes of mice bearing tumor in experiment group were increased(P
