Outcome of amniotic membrane transplanted under rabbit conjunctiva
- VernacularTitle:羊膜移植于结膜下的转归
- Author:
Na LI
- Publication Type:Journal Article
- Keywords:
Amniotic membrane transplantation;
Conjunctiva;
Turnover
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the turnover and influencing factors for amniotic membrane transplantation under conjunctiva. Methods:Amniotic membrane stained with DTAF was implanted under conjunctiva of 20 New Zealand white rabbits. After transplantation,we observed the clinical change. The tissue samples from grafted area were observed by HE staining and under laser confocal micro-scope to trace amniotic membrane on 3,5,8,12,16 W after surgery. Results:The amniotic membrane was intact until 3W after trans-plantation. The amniotic membrane was at the bottom of the conjunctiva and fascia,near the top of the sclera. At the 5th week after surgery,the amniotic membrane was bandshaped and began dissolved. Remnants of the amniotic membrane could be identified at the 8th week after surgery. After 12 W,the amniotic membrane basically dissolved,but remnants of curmbly erythroic tissue were locally left. The amniotic membrane totally dissolved after 16W. The types of inflammatory cells were mainly plasma cells,mononucleimacrophages and lymphocytes. At the 3th week after surgery,the inflammatory reaction reached the peak,then the inflammatory cells decreased. The main pathologic reaction was chronic inflammation.Laser confocal microscope discovered that the amniotic membrane persisted for 12 weeks under conjunctiva. Conclusion:The amniotic membrane implanted under conjunctiva persisted for 12 weeks and finally dissolved.
