HPLC fingerprinting of flavones in Gynostemma pentaphyllum
- VernacularTitle:绞股蓝药材黄酮类化合物的HPLC指纹图谱研究
- Author:
Fang LIU
- Publication Type:Journal Article
- Keywords:
Gynostemma pentaphyllum;
flavone;
HPLC fingerprint
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a HPLC fingerprint of flavones in Grynostemma pentaphyllum for the quality evaluation. METHODS: To extract flavones in G.pentaphyllum by reflux with methanol.After being optimized the HPLC condition,the fingerprint of flavones in G.pentaphyllum was developed and validated.10 batches of G.pentaphyllum were then analysed. RESULTS: 7 peaks in fingerprint profile were selected as characteristic peaks for validation.In precision,stability and repeatability studies,the RSDs of relative retention time and relative peak area were less than 0.5% and 5%,respectively.Similarities of the 10 batches of G.pentaphyllum were more than 0.8. CONCLUSION: The HPLC fingerprint of flavones in G.pentaphyllum can be used for quality evaluation of flavones in G.pentaphyllum.
