Identification of compounds in supercritical CO_2 extraction from Hypericum Perforatum L. by HPLC
- VernacularTitle:贯叶连翘超临界提取物的高效液相色谱分析
- Author:
Yanping LUO
- Publication Type:Journal Article
- Keywords:
HPLC;
Hypericum perforatum L.;
CO_2 supercritical fluid extraction/SFE-CO_2
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To identify and determine compounds in supercritical CO_2 extraction from Hypericum perforatum L. METHODS: The contents of flavones and hyperforin were determined by reverse phase HPLC.The condition in supercritical fluid extraction(SFE) was: extraction temperature at 40 ?C,extraction pressure at 20 MPa,separation temperature at 45 ?C,separation presure at 8 MPa,extraction time 1 h. RESULTS: There was no flavones and the content of hyperforin was 180.6 mg/g in the sample of SFE using neat CO_2.The flavones and hyperforin were found in SFE extract using ethanol as modifier and the contents of rutin,quercetin,hyperoside,hyperforin were 1.10 mg/g、1.84 mg/g、1.33 mg/g、26.19 mg/g,respectively. CONCLUSION: The concentration of hyperforin by SFE with neat CO_2 is significantly higher than that with modified supercritical CO_2 extraction. The method of SFE with neat CO_2 can be used to prepare extracts with high concentration hyperforin.
