Effects of Embedding Medicine in Acupoints and Electro-acupuncture on Expression of HSP70 in Hippocampus of Rat with Focal Cerebral Ischemia Reperfusion
- VernacularTitle:穴位埋药线和电针干预对脑缺血再灌注大鼠海马热休克蛋白70表达的影响
- Author:
Lihong KONG
;
Shan GAO
;
Zhenjiang CHEN
- Publication Type:Journal Article
- Keywords:
cerebral ischemia reperfusion;
Tetramethylpyrazine;
embedding medicine in points;
electro-acupuncture;
rat hippocampus;
HSP-70
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of embedding implantation of collagen and Tetramethylpyrazine in acupoints and electro-acupuncture on the expression of HSP70 in hippocampus of rat with focal cerebral ischemia reperfusion. Methods SD rats were randomly divided into normal group, sham operation group, model group, electro-acupuncture group, embedding medicine in acupoints group. Model of local ischemia was made by middle cerebral artery occlusion, preparation of implantation collagen and electro acupuncture and Tetramethylpyrazine was embedded in the acupoints of Dazhui and Neiguan two-sided. The expression of HSP-70 protein in hippocampus of different time point (24, 72, 120 h) was assayed by immunohistochemistry. Results There were only few positive cells of HSP-70 in normal group and sham operation group. The expression of HSP-70 in each therapy group were all heightened, and achieved the peak 24 h later and then declined in brain tissue after focal cerebral ischemiareperfusion, the differences were relatively prominent compared with the same phase model group (P
