Determination of 4’-O-?-D-glucosyl-5-O-methylvisamminol in Ganmao Qingre Granule by HPLC
- VernacularTitle:高效液相色谱法测定感冒清热颗粒中5-0-甲基维斯阿米醇苷的含量
- Author:
Ling LI
- Publication Type:Journal Article
- Keywords:
Ganmao Qingre Granule;
4’-O-?-D-glucosyl-5-O-methylvisamminol;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining the content of 4’-O-?-D-glucosyl-5-O methylvisamminol in Ganmao Qingre Granule by HPLC. Method The seperation was performed in Hypersil C18 colunm (4.6 mm?250 mm, 5 ?m) with the mobile phase of methanol-water (44∶56). The flow rate was 1.0 mL/min and the wavelength was set at 292 nm. Results The calibration curves was linear in the range of 4.608~46.08 ?g/mL for 4’-O-?-D-glucosyl-5-O-methylvisamminol (r =0.999 9). The average recovery was 97.9%, RSD=0.71% (n=6). Conclusion The method is simple, reproducible and can be used for the quality control of Ganmao Qingre Granule.