Determination of Astraloside in Xingnao Zaizao Capsules by HPLC-ELSD
- VernacularTitle:HPLC-ELSD测定醒脑再造胶囊中黄芪甲苷的含量
- Author:
Yan LI
;
Qingmiao WU
;
Yanyu ZHU
- Publication Type:Journal Article
- Keywords:
Xingnao Zaizao Capsules;
Astraloside;
HPLC-ELSD;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quantitative method for Astraloside in Xingnao Zaizao Capsules by HPLC-ELSD. Methods HPLC-ELSD separation was achieved on Phenomenex Gemini C18 column (250 mm?4.60 mm, 5 ?m) and mobile phase consisted of acetonitrile-water (36∶64). The flow rate was 1.0 mL/min, drift tube temperature was 105 ℃, and gas flow rate was 2.7 L/min. Results The calibration curve was linear in the range of 0.486～2.43 ?g (r=0.999 7). The average recovery rate was 100.5% (n=6), RSD was 0.3%. Conclusion The method is rapid, accurate and reliable, and the method can be used as the quality standard for Xingnao Zaizao Capsules.
