Determination of Quercetinum in Kuding Cha of Ligustrum by HPLC
- VernacularTitle:高效液相色谱法测定木樨科苦丁茶中槲皮素的含量
- Author:
Yiwen CHEN
;
Xianrong LI
;
Yan NI
- Publication Type:Journal Article
- Keywords:
Kuding Cha of Ligustrum;
quercetinum;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up the determining method of quercetinum in Kuding Cha of Ligustrum by HPLC. Method Hypersil ODS2 chromatographic column (4.6 mm?250 mm, 5 ?m) was adopted. Methanol 0.4% phosphoric acid (47∶53) was used as mobile phase, the flow rate was 1 mL/min, and the detecting wavelength was 360 nm. Result The linear range of quercetinum was 0.050 4～1.008 0 ?g, r =0.999 99, and the average recovery was 101.61% (RSD=1.75%, n =5). Conclusion The method is convenient and accurate, and can used be as basis of quality control for Kuding Cha of Ligustrum.
