Isolation and Purification of Vinblastine and Vincristine from Gatharanthus Roseus by Macroporous Adsorption Resin
- VernacularTitle:大孔吸附树脂分离纯化长春花中长春碱和长春新碱
- Author:
Yuefeng PAN
;
Huimin WANG
;
Yue ZHANG
- Publication Type:Journal Article
- Keywords:
macroporous adsorption resin;
Gatharanthus Roseus;
vinblastine;
vincristine
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the technological parameters of separation and purification of vinblastine and vincristine from Gatharanthus Roseus. Methods Different types of macroporous adsorption resin were used to separate and purify vinblastine and vincristine from Gatharanthus Roseus, eluting with different concentration of alcohol aqueous and velocities, combined with silica gel column chromatography, the process was monitored by HPLC. Results AB-8 type resin showed better comprehensive adsorption property, 90% alcohol aqueous and 1.5 BV/h velocities were used to elute. Ratio adsorption quantity was achieved to 74.5 mg/g. Through silica gel column chromatography, the purity of vincristine was achieved to 97.26% and the purity of vinblastine was achieved to 94.18%. Conclusions The process is simple and reasonable with good reproducibility. It is effective to enrich highly purified vinblastine and vincristine.
