Effects of salidroside on signal transduction of Rho/ROCK in the hepatic stellate cell
- VernacularTitle:红景天甙对HSC-T6细胞株Rho/ROCK信号通路影响的研究
- Author:
Jinfeng WU
- Publication Type:Journal Article
- Keywords:
Salidroside;
HSC-T6;
Rho/ROCKsignaltransductionpathway
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of salidroside on the proliferation,activation,Collagen Ⅰ secretion as well as expression of ROCK-Ⅰand TIMP-1 in HSC-T6 cell,and to elucidate the molecular mechanism of salidroside against hepatic fibrosis with Y-27632 as the positive control. Methods: HSC-T6 cell line was employed as the model for following study. MTT colorimetric assay was used to detect the proliferation of HSC-T6 cell. ELISA was employed to value the content of collagen Ⅰ in supernatant of HSC-T6 cells treated with salidroside and Y-27632. The effects of salidroside and Y-27632 on expressions of smooth muscle actin (?-SMA)、ROCK-Ⅰand TIMP-1 expression were analyzed by Real-time PCR and Western blot. Results(:1)Both Salidroside(3、6、12 mmol/L)and Y-27632(5、 10、20 ?mol/L)had dose-dependent inhibitory effects on ?-SMA expression which represents the activated state of HSC-T6(P
