Preliminary experimental study of the effects of vitreous cryopreservation with tetramethylpyrazine on schwann’s cell apoptosis and the expression of regulating gens
- VernacularTitle:含川芎嗪的玻璃化保存液对雪旺细胞凋亡及调控基因表达影响的初步实验研究
- Author:
Jianwen LIU
- Publication Type:Journal Article
- Keywords:
Ttetramethylpyrazine;
Schwann’s cell;
Vitreous cryopreservation;
Cell apoptosis
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To investigate the effects of vitreous cryopreservation with tetramethylpyrazine on schwann’s cell apoptosis and the expression of regulating gens under differenttemperatures. Methods:48 sciatic nerves obtained from 24 SD rats were randomly divided into 2 groups (each with 24 nerves): experimental group(with tetramethy -lpyrazine,320mg/L)and control group(no tetramethylpyrazine). Experimental group included 4 subgroups :A B C D;and the control group included 4 subgroups: A’,B’,C’,D’(each with 6 nerves). All the corresponding subgroups werecryopreserved3weeksunderdifferenttemperature(sAA’:-4℃,BB’:-20℃,CC’:-80℃,DD’:-196℃).After 3 weeks,all the nerves were obtained for tests: ①HE staining for light-microscope test,② Schwann’S cell apoptosis was measured quantitatively by the terminal transferase UTP nick end labeling (TUNEL)method,③The expression of Bcl-2 and Bax was determined by immunohistochemisty technique. Results:Aside from D D’subgroups,the number of Schwann’s cell apoptosis in each experimental subgroup was significantly smaller than that in the corresponding control subgroup(P0.05). Conclusion:These findings suggest that at -20℃ vitreous cryopreservation with TMP can depress Schwann’S cell apoptosis of rat by regulating the expression of Bcl-2 and Bax.
